Transfection of siRNAs into CML primary cells using Nucleofection®
Amaxa GmbH
Date Posted: Friday, May 23, 2008
RNA interference studies are based on transfection of short oligonucleotides (siRNAs) into cells and transfection efficiency is a crucial factor for such experiments. Most conventional transfection methods are suitable for immorta lized cell lines, but are not efficient in primary cells. We performed comparative analysis of transfection methods for siRNA delivery into peripheral blood leukocytes from chronic myeloid leukemia (CML) patients. In conclusion, transfection by amaxa Nucleofector® Technology represented the best and only suitable non-viral method for transfection of CML primary cells.
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