Search for a poster by keyword

Highly Efficient High-Throughput Transfection
Markus Zumbansen1, Allison St. Amand2, Devin Leake2, Ludger Altrogge1, and Herbert Müller-Hartmann1. 1 amaxa AG. 2 Dharmacon Inc.

Successful RNAi experiments and large-scale siRNA screens require efficient delivery of highly functional and specific nucleic acids including siRNA oligonucleotides, shRNA vectors, or micro RNAs into an appropriate cell system. Cell types relevant for immunological research, such as primary T cells and several suspension cell lines, are poorly accessible using reagent-based transfection approaches.

Critical factors for successful RNAi experiments in primary cells and hard to transfect cell lines
Markus Zumbansen1, Nicole Spottke1, Sheila Offizier1, Allison St. Amand2, Devin Leake2, Ludger Altrogge1, Meike Weigel1, Sandra Domzalski1, Dietmar Lenz, and Herbert Müller-Hartmann1. 1 amaxa AG, Cologne, Germany, D-50829 2 Dharmacon Inc., Thermo Fisher Scientific, Lafayette, CO 80026

The amaxa Nucleofector® Technology is a well established method for effective, non-viral transfection of any nucleic acid substrate into hard-to-transfect cells, especially suspension and primary cells. With the Nucleofector® 96-well Shuttle® system high throughput applications such as siRNA-library screenings have become amenable for the first time in these cell types. This renders target validation and identification possible in cell types highly relevant for biomedical research. Here we dis

Diagnostic Significance of microRNA Tumor Biomarkers in Fine Needle Aspirates (FNA) of Pancreatic Cancer
Martina Doleshal 1, Anna E. Szafranska 1, Hayward S. Edmunds 2, Gregory J. Tsongalis 2, Murray Korc 2 and Emmanuel Labourier 1. Asuragen, Inc., Dartmouth Medical School and Dartmouth Hitchcock Medical Center

Collection of pancreatic fine needle aspirates (FNAs) in RNARetain™ allows stabilization and protection of RNA in tissues containing high levels of nucleases; Differential miRNA expression in pancreatic FNAs accurately classifies benign and malignant tissue; Combinations of miRNA and mRNA expression signatures improve the separation between normal tissue and chronic pancreatitis; microRNAs are suitable analytes for molecular characterization of fine needle aspirates from pancreatic tissues.

Apoptosis Inducing Novel microRNA for Breast cancer and Hepatocellular Carcinoma
Arutselvan Natarajan, Sally J. DeNardo, Mark A. Zern, Senthil K. Venugopal. UC Davis Medical Center

MicroRNA provides a mechanism to target cancer stem cells and restraint tumors cells permanently; this could lead to promising cancer therapeutics and imaging agents. Apoptosis inducing microRNA was identified to target HCC and breast cancer cells for imaging and therapy.

A genome-wide loss of function screen identifies new genes required for lung cancer cell proliferation
PINNA G, ARAUJO N, MOROZOVA N, DETREZ MA & HAREL-BELLAN A. CNRS

RNA interference (RNAi) provides an experimental tool for functional genomics analysis. We screened a genome-wide RNAi library to identify new genes involved in lung cancer cell proliferation. 72 % from the 257 genes identified were involved in general gene expression processes, 16 % were of unknown function or unrelated to proliferation, and 12% consisted of uncharacterized genes. These last sets of genes provide potential novel targets for lung cancer treatment.

Computational analysis of high-throughputsmall RNA sequencing reveals microRNAs in a single-celled organism
Schwach F, Studholme DJ, Molnar A, Thuenemann E, Burrell M, Perez-Martinez A, Moulton V and Baulcombe D. University of East Anglia, Sainsbury Laboratory

We present evidence that miRNA precursors are present in the single-celled green alga Chlamydomonas reinhardtii and that they give rise to mature miRNAs that regulate target genes by post-transcriptional RNA cleavage.

 1 - 6 of 37 Next